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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/04/2021 |
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Data da última atualização: |
13/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, T. P. de; CHAIBUB, A. A.; CORTES, M. V. de C. B.; BATISTA, T. F. C.; BEZERRA, G. de A.; SILVA, G. B. da; FILIPPI, M. C. C. de. |
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Afiliação: |
THATYANE PEREIRA DE SOUSA, UFG; AMANDA ABDALLAH CHAIBUB, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; TELMA FATIMA COELHO BATISTA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; GUSTAVO DE ANDRADE BEZERRA, UFG; GISELE BARATA DA SILVA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
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Título: |
Molecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Archives of Microbiology, 2021. |
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ISSN: |
0302-8933 |
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DOI: |
https://doi.org/10.1007/s00203-021-02307-5 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process. MenosThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M... Mostrar Tudo |
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Palavras-Chave: |
Antagonism; Bioagent; Mycoparasitism. |
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Thesagro: |
Antagonismo; Arroz; Brusone; Controle Biológico; Marcador Molecular; Oryza Sativa; Trichoderma. |
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Thesaurus Nal: |
Biological control; Mycoparasites; Rice. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02690naa a2200373 a 4500
001 2131247
005 2021-04-13
008 2021 bl uuuu u00u1 u #d
022 $a0302-8933
024 7 $ahttps://doi.org/10.1007/s00203-021-02307-5$2DOI
100 1 $aSOUSA, T. P. de
245 $aMolecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast.$h[electronic resource]
260 $c2021
520 $aThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process.
650 $aBiological control
650 $aMycoparasites
650 $aRice
650 $aAntagonismo
650 $aArroz
650 $aBrusone
650 $aControle Biológico
650 $aMarcador Molecular
650 $aOryza Sativa
650 $aTrichoderma
653 $aAntagonism
653 $aBioagent
653 $aMycoparasitism
700 1 $aCHAIBUB, A. A.
700 1 $aCORTES, M. V. de C. B.
700 1 $aBATISTA, T. F. C.
700 1 $aBEZERRA, G. de A.
700 1 $aSILVA, G. B. da
700 1 $aFILIPPI, M. C. C. de
773 $tArchives of Microbiology, 2021.
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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| Registros recuperados : 12 | |
| 3. |  | FRANCISCON, L.; RIBEIRO JUNIOR, P. J.; KRAINSKI, E. T.; BASSANEZI, R. B.; CZERMAINSKI, A. B. C. Modelo autologístico espaço-temporal com aplicação à análise de padrões espaciais da Leprose-dos-citros. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 12, p. 1677-1682, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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| 6. | | BASSANEZI, R. B.; CZERMAINSKI, A. B. C.; KRAINSKI, E. T.; RIBEIRO JÚNIOR, P. J.; LARANJEIRA, F. F.; AMORIM, L. Progresso espaço-temporal da leprose dos citros. In: WORKSHOP DE EPIDEMIOLOGIA DE DOENÇAS DE PLANTAS, 2., 2007, Campos do Jordão. [Anais...] [Piracicaba: ESALQ, 2007]. p. 52-57.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 7. | | BOGNOLA, I. A.; RIBEIRO JÚNIOR, P. J.; SILVA, E. A. A. da; LINGNAU, C.; HIGA, A. R. Modelagem uni e bivariada da variabilidade espacial de rendimento de Pinus taeda L. Floresta, Curitiba, v. 38, n. 2, p. 373-385, abr./jun. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Florestas. |
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| 8. | | GREGO, S.; GREGO, C. R.; RIBEIRO JUNIOR, P. J.; VAZ, C. M. P.; HURTADO, S.; RODRIGUES, K. DE M. Densidade do solo de um Nitrossolo sob cultivo de cana-de-açúcar utilzando analises geoestatística e bayesiana. In: SIMPÓSIO DE GEOESTATÍSTICA APLICADA EM CIÊNCIAS AGRÁRIAS, 3., 2013, Botucatu, SP. Anais... Botucatu, SP: 4 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| 9. | | SAUTTER, K. D.; MOTTA NETO, J. A.; MORAES, A. de; SANTOS, H. R. dos; RIBEIRO JUNIOR, P. J. População de Oribatei e Collembola em pastagens na recuperação de solos degradados pela mineração do xisto. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 33, n. 9, p. 1509-13, set. 1998. Título em inglês: Population of Oribatei and Collembola in pastures for reclamation of degraded lands in a schist mine.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | PEIXOTO, A. P. B.; SANTANA, T. V. F. de; RIBEIRO JUNIOR, P. J.; OLIVEIRA, M. C. N. de; MACEDO, J. R. de; CAPECHE, C. L. Aplicação de métodos geoestatísticos bayesianos na análise de dados composicionais. In: SIMPÓSIO NACIONAL DE PROBABILIDADE E ESTATÍSTICA, 19., 2010, São Pedro, SP. [São Paulo]: Associação Brasileira de Estatística, 2010. 5 p. SINAPE.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | COLDEBELLA, A.; MACHADO, P. F.; DEMÉTRIO, C. G. B.; RIBEIRO JÚNIOR, P. J.; CORASSIN, C. H.; MEYER, P. M.; CASSOLI, L. D. Contagem de células somáticas e produção de leite em vacas holandesas de alta produção. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 12, p. 1451-1457, dez. 2003 Título em inglês: Somatic cells count and milk yield in high production Holstein cows.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 12. | | BASSANEZI, R. B.; CZERMAINSKI, A. B. C.; BARBOSA, F. F. L.; MOREIRA, A. S.; RIBEIRO JUNIOR, P. J.; KRAINSKI, E. T.; AMORIM, L. Spatial patterns of the Citrus leprosis virus and its associated mite vector in systems without intervention. Plant Pathology, 2018.[online] Publicação impressa em: Plant Pathology, v. 68, p. 85-93, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Uva e Vinho. |
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| Registros recuperados : 12 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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